Start • Life Sciences And Research Solutions • Innovation Partnerships • Advanced Cell Diagnostics (ACD), a Bio-Techne brand Advanced Cell Diagnostics (ACD), a Bio-Techne brand Image ACD - a Bio-Techne brand ACD - a Bio-Techne brand, is a pioneer and leader in the advancement of spatial genomics by unlocking the power of RNA and developing cell- and tissue-based research. Based on proprietary technology, ACD’s RNAscope Technology, the first multiplex fluorescent and chromogenic in situ hybridization platform enables detection and quantification of RNA in situ at single molecule sensitivity. Features and Benefits of Our Technology Image Features Strong detection of low expressors- Visualization of even very low expressing genes in any of the three probe channels. Compatible with multiple sample types- Detection of signals at low exposure and supports of FFPE samples and fresh frozen samples. Supports dual ISH and IHC protocols- High and stable signals enable sequential detection of proteins by IHC on the same samples. Sharper, crisper signal- Strong balanced signals across all three channels make it easy to generate publication-quality data. Image Legend: Mouse Brain Cortex GFAP (Green) (Antibody); Rbfox3 (red) ; Aif1 Benefits Easy to use - no need to match expression level of targets with channel sensitivity Novel multiplex nucleic acid ISH technology with a patented probe design Delivers clear and actionable results with high sensitivity and specificity Research Focus Automated Protocol - Best-in-class RNA ISH for your automated system of choice For labs that require high throughput and automated RNA in situ hybridization (ISH) analysis, ACD has partnered with Leica Biosystems to deliver RNAscope™ ISH automated assays. Common applications include studies to map co-expression of targets within the same cellular context (e.g. secreted ligand and its receptor) or to profile gene expression in a specific cell type expressing a known marker (e.g. a specific stem cell marker). Image Grin2C (dark brown) and Pval (red) detection in mouse brain using RNAscope™ 2.5 LS and BOND RX. Custom Protocol Using proprietary ACD RNAscope™ Probe Design Pipeline, we provide double "Z" oligo probes designed to hybridize to your specific RNA target of interest. We can design in situ hybridization RNA probes for virtually ANY gene in ANY genome for visualization in ANY tissue or cell. Image Sample Images Based on Kits RNAscope™ 2.5 LS Reagent Kit – BROWN Image RNAscope™ 2.5 LS Brown - Breast Cancer - FGFR3 RNAscope™ 2.5 LS Reagent Kit – RED Image RNAscope™ 2.5 LS Red - Head & Neck Cancer - HPV16 RNAscope™ 2.5 LS Duplex Assay Image Detection of Grin2C (dark brown) and Pvalb (red) mRNA in mouse brain tissue with RNAscope™ 2.5 LS Duplex Assay (RNA ISH) RNAscope™ LS Multiplex Fluorescent Assay Image RNA expression of Sc17a6-C1 (Vglut2) (green), Slc17a7-C2 (Vlgut1) (red) and Slc32a1-C3 (Vgat) in mouse brain fresh frozen tissue using RNAscope LS Multiplex Fluorescent Assay BaseScope™ LS Reagent Kit – RED Image BaseScope LS- Ovarian Cancer- TNFa-3EJ miRNAscope™ LS Reagent Kit Red Image miRNAscope™ + IHC detection of myeloid-specific miR-223-3p (red staining) and T-cell marker CD3 (green staining) in human cervical cancer tissue. The ISH-IHC staining shows mutually exclusive localization of these two targets Meet Our People Image Brad Dirks Director, Global Partnerships & Strategic Alliances Brad Dirks leads the Spatial Biology Division’s efforts to build and maintain strong relationships with our external partners to deliver “best-in-class” assays to researchers around the world. Image Rhonda Henshall-Powell Ph.D., Marketing Manager Rhonda’s experience in Digital Product and Social Media Marketing, and Strategic Key Opinion Leader relationships drives global sales and marketing teams to bring new products and raise brand awareness in the Spatial Biology Markets. miRNAscope™ A novel in situ hybridization technology for the detection of small regulatory RNAs with spatial and morphological context Image Download Now Publications Semiquantitative Methods for GFP Immunohistochemistry and in situ Hybridization to Evaluate AAV Transduction of Mouse Retinal Cells Following Subretinal Injection Publication Year: 2021 Country: USA Publication: Bondarenko G, Sorden SD, Christian BJ, Webster S, Sharma AK. Semiquantitative Methods for GFP Immunohistochemistry and In Situ Hybridization to Evaluate AAV Transduction of Mouse Retinal Cells Following Subretinal Injection. Toxicol Pathol. 2021;49(3):537-543. doi:10.1177/0192623320964804 Institute: Covance Laboratories Inc Instrument: BOND RX, RNAscope ISH assay, RNAscope 2.5 LS Reagent Kit—RED Authors: Bondarenko G, Sorden SD, Christian BJ, Webster S, Sharma AK. Study Goals: To develop methods for the evaluation of green fluorescent protein (GFP) and GFP transcript biodistribution in paraformaldehyde-fixed paraffin-embedded (PFPE) eye sections to assess the effectiveness of Adeno-associated virus (AAV) gene delivery in an experimental ocular toxicity study Keywords: adeno-associated virus vector, green fluorescent protein, immunohistochemistry, in situ hybridization, ocular toxicology, retina, gene therapy Detection of Engineered T Cells in FFPE Tissue by Multiplex in situ Hybridization and Immunohistochemistry Publication Year: 2021 Country: USA Publication: Wright JH, Huang LY, Weaver S, et al. Detection of engineered T cells in FFPE tissue by multiplex in situ hybridization and immunohistochemistry. J Immunol Methods. 2021;492:112955. doi:10.1016/j.jim.2020.112955 Institute: Fred Hutchinson Cancer Research Center 4Department of Immunology, University of Washington School of Medicine Instrument: BOND RX, Leica Dewax Solution, Epitope Retrieval Solution 2 , BOND Polymer Refine DAB Detection, (ACD) Enzyme (Proteinase III), ACD target probe, WPRE, positive control probes for mouse, human peptidyl-prolyl cis-trans isomerase B (PPIB; ACD #322100), negative control probe 4-hydroxy-tetrahydrodipicolinate reductase (DapB; ACD #312038), RNAscope 2.5 LS Reagent Kit-Brown Authors: Wright JH, Huang LY, Weaver S, et al. Study Goals: To develop a method for characterizing engineered T cells in fixed tissue using in situ hybridization (ISH) to the woodchuck hepatitis post-transcriptional regulatory element (WPRE) Keywords: CAR-T, TCR-T, in situ hybridization, Immunohistochemistry, WPRE, Immunophenotyping For Research Use Only. Not for use in diagnostic procedures.
