Knowledge-Pathway-Hero-image

The Benefit Of Multiple Tissue Processing Runs

Ashley Troutman
Ashley Troutman MBA, HT(ASCP), QIHC, CLSSBB.

Tissue processing is one of the most critical elements of obtaining quality and timely results in the histology process. Tissue processing in the laboratory prepares a specimen by infiltrating the tissue with paraffin wax to cut into thin slices in preparation for staining. Fixatives, processing protocols, tissue type, and size are critical factors for optimal tissue processing results.1 According to Frieda Carson, the biggest problem when processing tissue is processing both biopsy and large [routine] tissue specimens in the same processing run.2 Tissue can become over-processed – that is, the tissue may appear to be cooked, brittle, crisp, dry, or powdery, or under-processed and cannot be sectioned. Biopsy and larger sample tissues should be processed separately using a protocol specific to the specimen.3

Method

This study, conducted by the Leica Biosystems Content and Evidence Team, occurred at a small independent laboratory. The laboratory was interested in a complete review of their current processes and recommendations for quality and turn-around time improvements. As a result of the initial observations, the team focused on the laboratory’s tissue processing practices.

Results

one

Discussion

The Leica Biosystems Content and Evidence Team examined the laboratory’s current tissue processing schedule.  The laboratory uses a single tissue processor with one retort for one overnight batch run for all specimen types, including biopsies and routine tissue. Highly variable sizes in biopsy tissue versus routine tissue, and even fatty tissue, require significant time changes in the processing reagents. Combining tissue types into one overnight batch run can result in over-processing biopsies while not fully processing larger routine tissue. Tissue that is not initially processed sufficiently is pulled out of the daily workload and reprocessed on the next overnight processing run, delaying turn-around time on those cases by more than 24 hours.

As a result of this assessment, the recommendation is to separate processing schedules to accommodate the various tissue types and sizes:

  • Run #1 overnight for 12 hours for the routine specimens
  • Run #2 (optional) during the day for 2 hours for biopsies
  • Run #3 a delayed run for biopsies received during the late afternoon 

To maintain the current workflow and the overlap of runs (that is, the delayed biopsy and 12-hour run), this processing model could only be accomplished by moving from 1 tissue processor to 2 tissue processors or from a single retort processor to a dual retort processor. 

Conclusion

Ultimately, to obtain the desired quality processed tissue samples, separate processing runs may be required appropriate to tissue size. The addition of a small biopsy run during the day, a delayed run for biopsies, and an overnight extended run for routine tissue can increase throughput and reduce turn-around time for biopsies. 

Projections and Realized Results are specific to the institution where they were obtained and may not reflect the results achievable at other institutions.

About the presenter

Ashley Troutman
Ashley Troutman , MBA, HT(ASCP), QIHC, CLSSBB.

Ashley Troutman has been involved in Laboratory Medicine for more than 20 years in clinical, research and administrative capacities. He has worked in facilities of all sizes, from small community hospitals and private labs to large academic medical centers and corporate reference labs. He has extensive experience in laboratory science and management, specifically in anatomic pathology and immunohistochemistry. He has managed routine histology operations and has been part of the team to aid researchers in designing experiments using histologic techniques. These roles have allowed Ashley to lead work process implementation teams that saw success in scientific innovation as well as improving laboratory efficiency through areas of waste/cost reduction, process improvement and safety.

Referenzen

Die Inhalte des Knowledge Pathway von Leica Biosystems unterliegen den Nutzungsbedingungen der Website von Leica Biosystems, die hier eingesehen werden können: Rechtlicher Hinweis. Der Inhalt, einschließlich der Webinare, Schulungspräsentationen und ähnlicher Materialien, soll allgemeine Informationen zu bestimmten Themen liefern, die für medizinische Fachkräfte von Interesse sind. Er soll explizit nicht der medizinischen, behördlichen oder rechtlichen Beratung dienen und kann diese auch nicht ersetzen. Die Ansichten und Meinungen, die in Inhalten Dritter zum Ausdruck gebracht werden, spiegeln die persönlichen Auffassungen der Sprecher/Autoren wider und decken sich nicht notwendigerweise mit denen von Leica Biosystems, seinen Mitarbeitern oder Vertretern. Jegliche in den Inhalten enthaltene Links, die auf Quellen oder Inhalte Dritter verweisen, werden lediglich aus Gründen Ihrer Annehmlichkeit zur Verfügung gestellt.

Vor dem Gebrauch sollten die Produktinformationen, Beilagen und Bedienungsanleitungen der jeweiligen Medikamente und Geräte konsultiert werden.

Copyright © 2024 Leica Biosystems division of Leica Microsystems, Inc. and its Leica Biosystems affiliates. All rights reserved. LEICA and the Leica Logo are registered trademarks of Leica Microsystems IR GmbH.

Wenn Sie sich dieses Schulungswebinar aus unserer monatlichen Reihe angesehen haben und dessen Anerkennung als Weiterbildung seitens der Sie zertifizierenden Organisation beantragen möchten, füllen Sie bitte das Formular aus, um Ihrem Protokoll die entsprechenden Weiterbildungspunkte gemäß Ihren eigenen Angaben hinzuzufügen.
ANMELDUNG FÜR WEITERBILDUNGSPUNKTE NACH EIGENANGABEN
Erhalten Sie neueste Informationen aus dem Knowledge Pathway direkt in Ihr Postfach.
Melden Sie sich jetzt an!
Wir sind stets auf der Suche nach weiteren interessanten Autoren, die wir hier vorstellen möchten. Reichen Sie Ihren Beitrag ein und wir melden uns bei Ihnen!
Senden Sie Ihren Beitrag
SHARE