Design, Optimization, and Validation of Multiplex Immunofluorescence Panels Michael J Surace Scientist II, AstraZeneca In this webinar, we will cover biological and technical considerations when designing a panel, a linear process for developing, testing, and optimizing a panel, and an approach for technical validation of multiplex panels which detects and addresses known risks. Multiplex immunofluorescence (mIF) combines the spatial information from immunohistochemistry ( IHC ) with multimarker phenotypes. Recent advances in mIF technology have made it possible for researchers to develop novel custom panels, but additional considerations must be taken into account in order to produce a panel which performs at least as well as IHC on a marker-by-marker basis, and avoids any undesirable interactions between detection of the targets and neighboring visible light spectra. Learning Objectives Attendees will learn about key factors to consider when designing a new mIF panel We will walk through an efficient process for developing and optimizing mIF panels using TSA-linked fluorophores and iterative staining Attendees will become familiar with the best use cases and advantages of most common mIF staining and imaging technologies Attendees will come away from the webinar with an adaptable scheme for testing and validating a novel mIF panel About the presenter Michael J Surace , Scientist II, AstraZeneca Michael Surace is a scientist at AstraZeneca, where he is responsible for the design, optimization, and validation of multiplex IF panels, as well as imaging and image analysis approaches specifically for the discovery of complex predicitive and prognostic biomarkers for immune oncology. Related Content Tips, Tricks, and Optimization: A User's Guide to BOND RX and Chromogenic Multiplexing in Research Applications Introduction to Antibodies Validating Antibodies for Research Applications in IHC Die Inhalte des Knowledge Pathway von Leica Biosystems unterliegen den Nutzungsbedingungen der Website von Leica Biosystems, die hier eingesehen werden können: Rechtlicher Hinweis. Der Inhalt, einschließlich der Webinare, Schulungspräsentationen und ähnlicher Materialien, soll allgemeine Informationen zu bestimmten Themen liefern, die für medizinische Fachkräfte von Interesse sind. Er soll explizit nicht der medizinischen, behördlichen oder rechtlichen Beratung dienen und kann diese auch nicht ersetzen. Die Ansichten und Meinungen, die in Inhalten Dritter zum Ausdruck gebracht werden, spiegeln die persönlichen Auffassungen der Sprecher/Autoren wider und decken sich nicht notwendigerweise mit denen von Leica Biosystems, seinen Mitarbeitern oder Vertretern. Jegliche in den Inhalten enthaltene Links, die auf Quellen oder Inhalte Dritter verweisen, werden lediglich aus Gründen Ihrer Annehmlichkeit zur Verfügung gestellt. Vor dem Gebrauch sollten die Produktinformationen, Beilagen und Bedienungsanleitungen der jeweiligen Medikamente und Geräte konsultiert werden. Copyright © 2024 Leica Biosystems division of Leica Microsystems, Inc. and its Leica Biosystems affiliates. All rights reserved. LEICA and the Leica Logo are registered trademarks of Leica Microsystems IR GmbH. RELATED PRODUCTS BOND RX Fully Automated Research Stainer BOND RXm RELATED TAGS AND TOPICS Automated IHC Workflow, Multiplex, Immunofluorescence, Validation RELATED CONTENT Tips, Tricks, and Optimization: A User's Guide to BOND RX and Chromogenic Multiplexing in Research Applications Introduction to Antibodies Validating Antibodies for Research Applications in IHC Contact Our Product Expert Now If you have viewed this educational webinar or training and would like to apply for continuing education credits with your certifying organization, please download the form to assist you in adding self-reported educational credits to your transcript. ANMELDUNG FÜR WEITERBILDUNGSPUNKTE NACH EIGENANGABEN Subscribe to our mailing list to learn about our upcoming symposiums and new research-focused product launches. Melden Sie sich jetzt an! SHARE Facebook Twitter LinkedIn Email
