Fundamentals of Hematoxylin and Eosin Staining Cindy Sampias JD CT(ASCP)HTL For characterizing tissue, the use of H&E is by far preferred for viewing cellular and tissue structure detail. The variation of stain intensity is often driven by the slide reviewers' learning experience and what they are comfortable seeing. Because this stain demonstrates a broad range of cytoplasmic, nuclear, and extracellular matrix features, nearly all teaching texts use H&E images. We continue to use this simple and essential stain today, which has remained unchanged for over a century. Learning Objectives Identify the four components of an H&E stain Describe the critical elements of developing an H&E staining protocol Define three characteristics of an optimal and suboptimal H&E stain In this webinar, Cindy Sampias, JD CT(ASCP)HTL, Applications Technical Team Lead - Core Histology at Leica Biosystems, provides an overview and discusses the practical uses of the Hematoxylin and Eosin stains gleaned from over 30 years of laboratory experience in the field of histology. During this presentation, Cindy provides insights and shares fundamental concepts about H&E staining, including the components, types, and “how-to” elements of developing protocols. Cindy shares “tips and tricks” with the audience on performing H&E staining optimizations and concludes by describing best practice examples of what to look for in optimal and suboptimal H&E stains. For Research Use Only. Not for Use in Diagnostic Procedures. About the presenter Cindy Sampias , JD CT(ASCP)HTL Cindy Sampias is a board certified Cyto- and Histo-technologist. With more than 25 years of experience, she is a guest speaker at histology and cytology meetings around the country. She is a technical author for Media Lab, publishing a variety of technical courses and sharing best practices in histology. Related Content H&E Image Quality & Tissue-based Research How to Create a High-Quality H&E Staining Protocol Die Inhalte des Knowledge Pathway von Leica Biosystems unterliegen den Nutzungsbedingungen der Website von Leica Biosystems, die hier eingesehen werden können: Rechtlicher Hinweis. Der Inhalt, einschließlich der Webinare, Schulungspräsentationen und ähnlicher Materialien, soll allgemeine Informationen zu bestimmten Themen liefern, die für medizinische Fachkräfte von Interesse sind. Er soll explizit nicht der medizinischen, behördlichen oder rechtlichen Beratung dienen und kann diese auch nicht ersetzen. Die Ansichten und Meinungen, die in Inhalten Dritter zum Ausdruck gebracht werden, spiegeln die persönlichen Auffassungen der Sprecher/Autoren wider und decken sich nicht notwendigerweise mit denen von Leica Biosystems, seinen Mitarbeitern oder Vertretern. Jegliche in den Inhalten enthaltene Links, die auf Quellen oder Inhalte Dritter verweisen, werden lediglich aus Gründen Ihrer Annehmlichkeit zur Verfügung gestellt. Vor dem Gebrauch sollten die Produktinformationen, Beilagen und Bedienungsanleitungen der jeweiligen Medikamente und Geräte konsultiert werden. Copyright © 2024 Leica Biosystems division of Leica Microsystems, Inc. and its Leica Biosystems affiliates. All rights reserved. LEICA and the Leica Logo are registered trademarks of Leica Microsystems IR GmbH. RELATED TAGS AND TOPICS Hematoxylin and Eosin RELATED CONTENT H&E Image Quality & Tissue-based Research How to Create a High-Quality H&E Staining Protocol Contact Our Product Expert Now If you have viewed this educational webinar or training and would like to apply for continuing education credits with your certifying organization, please download the form to assist you in adding self-reported educational credits to your transcript. ANMELDUNG FÜR WEITERBILDUNGSPUNKTE NACH EIGENANGABEN Subscribe to our mailing list to learn about our upcoming symposiums and new research-focused product launches. Melden Sie sich jetzt an! SHARE Facebook Twitter LinkedIn Email