Tissue Processing: A Cornerstone to Quality Downstream Testing Robin Fitzl Sr. Field Applications Specialist - Leica Biosystems Since the 19th century, the practice of tissue processing has remained largely unchanged, resulting in solidified, paraffin-embedded tissue blocks for sectioning. As staining procedures have advanced and immunohistochemistry is becoming more widely used, high-quality tissue sections are paramount for accurate interpretation of disease processes.. At each processing stage, many variables require consideration to eliminate artifacts and preserve tissue morphology to ensure high-quality sectioning. This talk will review basic strategies to develop best laboratory practices and protocols from the beginning of the histology workflow, which results in good quality sections to minimize the need for repeat downstream testing. Once the tissue has been processed well, getting good sections placed onto the glass slide should be easier; however, this is not always the case, with some cases proving challenging to cut. In this section of the talk, we will review the basics of embedding and sectioning, including paraffin types, molds, blades, and slides, as well as a discussion of artifacts. We will then present the “tricks of the trade” or “histology hacks” that almost every seasoned histologist has kept secret until now. For Research Use Only. Not for use in diagnostic procedures. Related Content Tissue Processing Artifacts: Checklist for Success Lessons From the Laboratory: Optimizing Your Tissue Processing Die Inhalte des Knowledge Pathway von Leica Biosystems unterliegen den Nutzungsbedingungen der Website von Leica Biosystems, die hier eingesehen werden können: Rechtlicher Hinweis. Der Inhalt, einschließlich der Webinare, Schulungspräsentationen und ähnlicher Materialien, soll allgemeine Informationen zu bestimmten Themen liefern, die für medizinische Fachkräfte von Interesse sind. Er soll explizit nicht der medizinischen, behördlichen oder rechtlichen Beratung dienen und kann diese auch nicht ersetzen. Die Ansichten und Meinungen, die in Inhalten Dritter zum Ausdruck gebracht werden, spiegeln die persönlichen Auffassungen der Sprecher/Autoren wider und decken sich nicht notwendigerweise mit denen von Leica Biosystems, seinen Mitarbeitern oder Vertretern. Jegliche in den Inhalten enthaltene Links, die auf Quellen oder Inhalte Dritter verweisen, werden lediglich aus Gründen Ihrer Annehmlichkeit zur Verfügung gestellt. Vor dem Gebrauch sollten die Produktinformationen, Beilagen und Bedienungsanleitungen der jeweiligen Medikamente und Geräte konsultiert werden. Copyright © 2024 Leica Biosystems division of Leica Microsystems, Inc. and its Leica Biosystems affiliates. All rights reserved. LEICA and the Leica Logo are registered trademarks of Leica Microsystems IR GmbH. RELATED PRODUCTS HistoCore PELORIS 3 Premium Tissue Processing System RELATED TAGS AND TOPICS Histology, Tissue Processing, Tissue Preparation, Quality RELATED CONTENT Tissue Processing Artifacts: Checklist for Success Lessons From the Laboratory: Optimizing Your Tissue Processing Contact Our Product Expert Now If you have viewed this educational webinar or training and would like to apply for continuing education credits with your certifying organization, please download the form to assist you in adding self-reported educational credits to your transcript. ANMELDUNG FÜR WEITERBILDUNGSPUNKTE NACH EIGENANGABEN Subscribe to our mailing list to learn about our upcoming symposiums and new research-focused product launches. Melden Sie sich jetzt an! SHARE Facebook Twitter LinkedIn Email
