Chromogenic Co-detection of Protein and mRNA Targets in Tissues using the BOND RX Fully Automated Research Stainer Bradley Spencer-Dene Subject Matter Expert, Next Gen Histology at GSK Co-detection of more than one target in Formalin-Fixed Paraffin Embedded samples is an extremely useful application and one that is increasingly required in modern histopathology labs. In order to detect both protein and mRNA targets in the same section, Bradley Spencer-Dene at GSK, has established a fully-automated workflow for sequentially detecting protein and mRNA targets chromogenically on the BOND RX research stainer combining AP/Fast Red detection (RNAscope/Basescope in situ hybridization) with HRP/Green detection (Immunohistochemistry) to establish target mRNA expression in the context of cell lineage detection. For Research Use Only. Not for use in diagnostic procedures. Related Content Digital Histopathology Deployment in GSK Non-Clinical Histology Facility The Power of Spatially Resolved Staining Enhanced Automated Multiplex Capabilities on Leica Biosystems BOND Research System Leica Biosystems content is subject to the Leica Biosystems website terms of use, available at: Legal Notice. The content, including webinars, training presentations and related materials is intended to provide general information regarding particular subjects of interest to health care professionals and is not intended to be, and should not be construed as, medical, regulatory or legal advice. The views and opinions expressed in any third-party content reflect the personal views and opinions of the speaker(s)/author(s) and do not necessarily represent or reflect the views or opinions of Leica Biosystems, its employees or agents. Any links contained in the content which provides access to third party resources or content is provided for convenience only. For the use of any product, the applicable product documentation, including information guides, inserts and operation manuals should be consulted. Copyright © 2024 Leica Biosystems division of Leica Microsystems, Inc. and its Leica Biosystems affiliates. All rights reserved. LEICA and the Leica Logo are registered trademarks of Leica Microsystems IR GmbH. RELATED PRODUCTS BOND RX Fully Automated Research Stainer BOND RXm RELATED TAGS AND TOPICS Automated IHC Workflow, Multiplex, Chromogenic Staining, Co-detection RELATED CONTENT Digital Histopathology Deployment in GSK Non-Clinical Histology Facility The Power of Spatially Resolved Staining Enhanced Automated Multiplex Capabilities on Leica Biosystems BOND Research System Contact Our Product Expert Now If you have viewed this educational webinar or training and would like to apply for continuing education credits with your certifying organization, please download the form to assist you in adding self-reported educational credits to your transcript. Apply for self-reported educational credits Subscribe to our mailing list to learn about our upcoming symposiums and new research-focused product launches. Subscribe today! SHARE Facebook Twitter LinkedIn Email
