Home • Knowledge Pathway • Training Resources • A Guide to Better IHC RELATED CONTENT Immunohistochemistry: An Overview + Steps to Better IHC Staining IHC Workflow Optimization Toolkit Technical Brief of Heat Induced Epitope Retrieval Eliminating Rework Through Utilization of Interfacing Automated Staining Platforms A Guide to Better IHC This guide helps you identify your staining issue and provides potential remedies to assist you in troubleshooting the staining issue quickly. Please keep in mind that some issues may be due to pre-staining conditions. Please refer to our 101 Steps to Better Histology guide for troubleshooting these pre-staining conditions. In this guide, you will learn how to identify and troubleshoot some of the following common issues with IHC staining: No antibody staining is present No staining and circular artifact Loss of staining after staining is completed Staining too weak Background staining on glass slides Staining too strong/appears overstained Background staining or non-specific staining Uneven or gradient staining Atypical chromogen/counterstain staining Tissue artifacts Additionally, receive 10 steps to optimizing your IHC staining. Download A Guide to Better IHC Now! Related Content Immunohistochemistry: An Overview + Steps to Better IHC Staining IHC Workflow Optimization Toolkit Technical Brief of Heat Induced Epitope Retrieval Eliminating Rework Through Utilization of Interfacing Automated Staining Platforms Leica Biosystems Knowledge Pathway content is subject to the Leica Biosystems website terms of use, available at: Legal Notice. The content, including webinars, training presentations and related materials is intended to provide general information regarding particular subjects of interest to health care professionals and is not intended to be, and should not be construed as, medical, regulatory or legal advice. The views and opinions expressed in any third-party content reflect the personal views and opinions of the speaker(s)/author(s) and do not necessarily represent or reflect the views or opinions of Leica Biosystems, its employees or agents. Any links contained in the content which provides access to third party resources or content is provided for convenience only. For the use of any product, the applicable product documentation, including information guides, inserts and operation manuals should be consulted. Copyright © 2024 Leica Biosystems division of Leica Microsystems, Inc. and its Leica Biosystems affiliates. All rights reserved. LEICA and the Leica Logo are registered trademarks of Leica Microsystems IR GmbH. If you have viewed this educational webinar, training or tutorial on Knowledge Pathway and would like to apply for continuing education credits with your certifying organization, please download the form to assist you in adding self-reported educational credits to your transcript. Apply for self-reported educational credits Get Knowledge Pathway updates delivered directly to your inbox. Subscribe today! We are looking for more great writers to feature here. Send us a submission and we’ll be in touch! Send your writing! SHARE Facebook Twitter LinkedIn Email
