How to Create a High-Quality H&E Staining Protocol Andrew Lisowski M.S., HTL (ASCP) The Hematoxylin and Eosin stain (H&E) is the most widely used stain for histopathological analysis. Even though the H&E stain is a relatively simple stain to perform, there are a variety of factors that can interfere with the staining process. The maintenance of consistent, high-quality H&E stains is a fundamental requirement in any histopathology related project. Troubleshooting H&E stains can be difficult and frustrating. Our objective is to highlight some of the most common mistakes when choosing staining reagents and creating protocols. By minimizing them, we can deliver high quality slides in the shortest possible time to improve the outcome of translational research projects. For Research Use Only. Not for use in diagnostic procedures. About the presenter Andrew Lisowski , M.S., HTL (ASCP) Andrew Lisowski has almost 30 years of experience in histology and histotechnology. He attended veterinary school and earned his master’s degree in molecular biology. Andrew worked in histology, IHC and ISH labs, cell culture lab, performed in-vitro and in-vivo toxicology assays and was a member of a necropsy team. He worked for pharmaceutical companies, medical school and founded his own molecular and histology firms. Related Content Fundamentals of Hematoxylin and Eosin Staining H&E Image Quality & Tissue-based Research Leica Biosystems content is subject to the Leica Biosystems website terms of use, available at: Legal Notice. The content, including webinars, training presentations and related materials is intended to provide general information regarding particular subjects of interest to health care professionals and is not intended to be, and should not be construed as, medical, regulatory or legal advice. The views and opinions expressed in any third-party content reflect the personal views and opinions of the speaker(s)/author(s) and do not necessarily represent or reflect the views or opinions of Leica Biosystems, its employees or agents. Any links contained in the content which provides access to third party resources or content is provided for convenience only. For the use of any product, the applicable product documentation, including information guides, inserts and operation manuals should be consulted. Copyright © 2024 Leica Biosystems division of Leica Microsystems, Inc. and its Leica Biosystems affiliates. All rights reserved. LEICA and the Leica Logo are registered trademarks of Leica Microsystems IR GmbH. RELATED PRODUCTS HistoCore SPECTRA Workstation Leica ST5010 Autostainer XL Leica CV5030 Fully Automated Glass Coverslipper RELATED TAGS AND TOPICS Histology, Primary Staining, Hemotoxin & Eosin, Quality RELATED CONTENT Fundamentals of Hematoxylin and Eosin Staining H&E Image Quality & Tissue-based Research Contact Our Product Expert Now If you have viewed this educational webinar or training and would like to apply for continuing education credits with your certifying organization, please download the form to assist you in adding self-reported educational credits to your transcript. Apply for self-reported educational credits Subscribe to our mailing list to learn about our upcoming symposiums and new research-focused product launches. Subscribe today! SHARE Facebook Twitter LinkedIn Email
