Tissue Processing: A Cornerstone to Quality Downstream Testing Robin Fitzl Sr. Field Applications Specialist - Leica Biosystems Since the 19th century, the practice of tissue processing has remained largely unchanged, resulting in solidified, paraffin-embedded tissue blocks for sectioning. As staining procedures have advanced and immunohistochemistry is becoming more widely used, high-quality tissue sections are paramount for accurate interpretation of disease processes.. At each processing stage, many variables require consideration to eliminate artifacts and preserve tissue morphology to ensure high-quality sectioning. This talk will review basic strategies to develop best laboratory practices and protocols from the beginning of the histology workflow, which results in good quality sections to minimize the need for repeat downstream testing. Once the tissue has been processed well, getting good sections placed onto the glass slide should be easier; however, this is not always the case, with some cases proving challenging to cut. In this section of the talk, we will review the basics of embedding and sectioning, including paraffin types, molds, blades, and slides, as well as a discussion of artifacts. We will then present the “tricks of the trade” or “histology hacks” that almost every seasoned histologist has kept secret until now. For Research Use Only. Not for use in diagnostic procedures. Related Content Tissue Processing Artifacts: Checklist for Success Lessons From the Laboratory: Optimizing Your Tissue Processing Leica Biosystems content is subject to the Leica Biosystems website terms of use, available at: Legal Notice. The content, including webinars, training presentations and related materials is intended to provide general information regarding particular subjects of interest to health care professionals and is not intended to be, and should not be construed as, medical, regulatory or legal advice. The views and opinions expressed in any third-party content reflect the personal views and opinions of the speaker(s)/author(s) and do not necessarily represent or reflect the views or opinions of Leica Biosystems, its employees or agents. Any links contained in the content which provides access to third party resources or content is provided for convenience only. For the use of any product, the applicable product documentation, including information guides, inserts and operation manuals should be consulted. Copyright © 2024 Leica Biosystems division of Leica Microsystems, Inc. and its Leica Biosystems affiliates. All rights reserved. LEICA and the Leica Logo are registered trademarks of Leica Microsystems IR GmbH. RELATED PRODUCTS HistoCore PELORIS 3 Premium Tissue Processing System RELATED TAGS AND TOPICS Histology, Tissue Processing, Tissue Preparation, Quality RELATED CONTENT Tissue Processing Artifacts: Checklist for Success Lessons From the Laboratory: Optimizing Your Tissue Processing Contact Our Product Expert Now If you have viewed this educational webinar or training and would like to apply for continuing education credits with your certifying organization, please download the form to assist you in adding self-reported educational credits to your transcript. Apply for self-reported educational credits Subscribe to our mailing list to learn about our upcoming symposiums and new research-focused product launches. Subscribe today! SHARE Facebook Twitter LinkedIn Email