Home • Knowledge Pathway • Tissue Processing Tissue Processing Tissue processing consists of dehydration, clearing, and infiltration. Here, experts share their recommendations for properly executing these steps. Image 101 Steps to Better Histology - a Practical Guide to Good Histology Practice Geoffrey Rolls, BAppSc, FAIMS This straightforward guide to good histology practice provides practical advice on best-practice techniques and simple ways to avoid common errors. Learn More Image Process of Fixation and the Nature of Fixatives Geoffrey Rolls, BAppSc, FAIMS Fixation is a critical step in the preparation of histological sections. If it is not carried out under optimal conditions or if fixation is delayed, a tissue specimen can be irreversibly damaged. No matter how much care is subsequently taken in tissue... Learn More Image Fixation and Fixatives (2) – Factors Influencing Chemical Fixation, Formaldehyde and Glutaraldehyde Geoffrey Rolls, BAppSc, FAIMS This second part of the Fixation and Fixatives series covers the factors that influence the rate and effectiveness of tissue fixation as well as looking at two common fixatives: formaldehyde (histology) and glutaraldehyde (ultrastructural electron microscopy... Learn More Image Fixation and Fixatives (3) – Fixing Agents Other than the Common Aldehydes Geoffrey Rolls, BAppSc, FAIMS As well as formaldehyde and glutaraldehyde, discussed previously, a number of other reagents have been used for fixation, sometimes in simple solution but often combined with other agents in the form of compound fixatives. The more important of these are... Learn More Image Fixation and Fixatives - Popular Fixative Solutions Geoffrey Rolls, BAppSc, FAIMS Fixation is a critical step in the preparation of histological sections. If it is not carried out under optimal conditions or if fixation is delayed, a tissue specimen can be irreversibly damaged. No matter how much care is subsequently taken in tissue... Learn More Image Fixation and Fixatives (5) – Practical Procedures to Optimize Quality, the Effects of Heat, and Microwaves Geoffrey Rolls, BAppSc, FAIMS In this final part of the Fixation and Fixatives series, we look at practical ways to optimize fixation quality, discuss how heat effects fixation, and finish with an introduction to the relatively new field of microwave fixation. Learn More Current page 1 Page 2 Page 3 Page 4
