Design, Optimization, and Validation of Multiplex Immunofluorescence Panels Michael J Surace Scientist II, AstraZeneca In this webinar, we will cover biological and technical considerations when designing a panel, a linear process for developing, testing, and optimizing a panel, and an approach for technical validation of multiplex panels which detects and addresses known risks. Multiplex immunofluorescence (mIF) combines the spatial information from immunohistochemistry ( IHC ) with multimarker phenotypes. Recent advances in mIF technology have made it possible for researchers to develop novel custom panels, but additional considerations must be taken into account in order to produce a panel which performs at least as well as IHC on a marker-by-marker basis, and avoids any undesirable interactions between detection of the targets and neighboring visible light spectra. Learning Objectives Attendees will learn about key factors to consider when designing a new mIF panel We will walk through an efficient process for developing and optimizing mIF panels using TSA-linked fluorophores and iterative staining Attendees will become familiar with the best use cases and advantages of most common mIF staining and imaging technologies Attendees will come away from the webinar with an adaptable scheme for testing and validating a novel mIF panel About the presenter Michael J Surace , Scientist II, AstraZeneca Michael Surace is a scientist at AstraZeneca, where he is responsible for the design, optimization, and validation of multiplex IF panels, as well as imaging and image analysis approaches specifically for the discovery of complex predicitive and prognostic biomarkers for immune oncology. Related Content Tips, Tricks, and Optimization: A User's Guide to BOND RX and Chromogenic Multiplexing in Research Applications Introduction to Antibodies Validating Antibodies for Research Applications in IHC El contenido de Leica Biosystems Knowledge Pathway está sujeto a las condiciones de uso del sitio web de Leica Biosystems, disponibles en: Aviso legal.. El contenido, incluidos los webinars o seminarios web, los recursos de formación y los materiales relacionados, está destinado a proporcionar información general sobre temas concretos de interés para los profesionales de la salud y no está destinado a ser, ni debe interpretarse como asesoramiento médico, normativo o jurídico. Los puntos de vista y opiniones expresados en cualquier contenido de terceros reflejan los puntos de vista y opiniones personales de los ponentes/autores y no representan ni reflejan necesariamente los puntos de vista ni opiniones de Leica Biosystems, sus empleados o sus agentes. Cualquier enlace incluido en el contenido que proporcione acceso a recursos o contenido de terceros se proporciona únicamente por comodidad. Para el uso de cualquier producto, debe consultarse la documentación correspondiente del producto, incluidas las guías de información, los prospectos y los manuales de funcionamiento. Copyright © 2024 Leica Biosystems division of Leica Microsystems, Inc. and its Leica Biosystems affiliates. All rights reserved. LEICA and the Leica Logo are registered trademarks of Leica Microsystems IR GmbH. RELATED PRODUCTS BOND RX Fully Automated Research Stainer BOND RXm RELATED TAGS AND TOPICS Automated IHC Workflow, Multiplex, Immunofluorescence, Validation RELATED CONTENT Tips, Tricks, and Optimization: A User's Guide to BOND RX and Chromogenic Multiplexing in Research Applications Introduction to Antibodies Validating Antibodies for Research Applications in IHC Contact Our Product Expert Now If you have viewed this educational webinar or training and would like to apply for continuing education credits with your certifying organization, please download the form to assist you in adding self-reported educational credits to your transcript. SOLICITAR CRÉDITOS EDUCATIVOS AUTODECLARADOS Subscribe to our mailing list to learn about our upcoming symposiums and new research-focused product launches. ¡Suscríbase hoy mismo! SHARE Facebook Twitter LinkedIn Email
