How to Create a High-Quality H&E Staining Protocol Andrew Lisowski M.S., HTL (ASCP) The Hematoxylin and Eosin stain (H&E) is the most widely used stain for histopathological analysis. Even though the H&E stain is a relatively simple stain to perform, there are a variety of factors that can interfere with the staining process. The maintenance of consistent, high-quality H&E stains is a fundamental requirement in any histopathology related project. Troubleshooting H&E stains can be difficult and frustrating. Our objective is to highlight some of the most common mistakes when choosing staining reagents and creating protocols. By minimizing them, we can deliver high quality slides in the shortest possible time to improve the outcome of translational research projects. For Research Use Only. Not for use in diagnostic procedures. About the presenter Andrew Lisowski , M.S., HTL (ASCP) Andrew Lisowski has almost 30 years of experience in histology and histotechnology. He attended veterinary school and earned his master’s degree in molecular biology. Andrew worked in histology, IHC and ISH labs, cell culture lab, performed in-vitro and in-vivo toxicology assays and was a member of a necropsy team. He worked for pharmaceutical companies, medical school and founded his own molecular and histology firms. Related Content Fundamentals of Hematoxylin and Eosin Staining H&E Image Quality & Tissue-based Research El contenido de Leica Biosystems Knowledge Pathway está sujeto a las condiciones de uso del sitio web de Leica Biosystems, disponibles en: Aviso legal.. El contenido, incluidos los webinars o seminarios web, los recursos de formación y los materiales relacionados, está destinado a proporcionar información general sobre temas concretos de interés para los profesionales de la salud y no está destinado a ser, ni debe interpretarse como asesoramiento médico, normativo o jurídico. Los puntos de vista y opiniones expresados en cualquier contenido de terceros reflejan los puntos de vista y opiniones personales de los ponentes/autores y no representan ni reflejan necesariamente los puntos de vista ni opiniones de Leica Biosystems, sus empleados o sus agentes. Cualquier enlace incluido en el contenido que proporcione acceso a recursos o contenido de terceros se proporciona únicamente por comodidad. Para el uso de cualquier producto, debe consultarse la documentación correspondiente del producto, incluidas las guías de información, los prospectos y los manuales de funcionamiento. Copyright © 2024 Leica Biosystems division of Leica Microsystems, Inc. and its Leica Biosystems affiliates. All rights reserved. LEICA and the Leica Logo are registered trademarks of Leica Microsystems IR GmbH. RELATED PRODUCTS HistoCore SPECTRA Workstation Leica ST5010 Autostainer XL Leica CV5030 Fully Automated Glass Coverslipper RELATED TAGS AND TOPICS Histology, Primary Staining, Hemotoxin & Eosin, Quality RELATED CONTENT Fundamentals of Hematoxylin and Eosin Staining H&E Image Quality & Tissue-based Research Contact Our Product Expert Now If you have viewed this educational webinar or training and would like to apply for continuing education credits with your certifying organization, please download the form to assist you in adding self-reported educational credits to your transcript. SOLICITAR CRÉDITOS EDUCATIVOS AUTODECLARADOS Subscribe to our mailing list to learn about our upcoming symposiums and new research-focused product launches. ¡Suscríbase hoy mismo! SHARE Facebook Twitter LinkedIn Email
