Accueil • Life Sciences And Research Solutions • Ressources pédagogiques • Articles • LS Articles • Tips & Tricks to Multiplexing: How to Choose Chromogen Colors for Multiplex and Detection Systems for Multiplex Assays RELATED PRODUCTS BOND RX Fully Automated Research Stainer BOND RXm RELATED TAGS AND TOPICS Automated IHC Workflow, Multiplex, Chromogenic Staining, Co-detection RELATED CONTENT Selecting the right detection for your IHC/ISH project (Fluorescence vs Chromogenic Staining) Design, Optimization, and Validation of Multiplex Immunofluorescence Panels Benefits Of Automation For Tissue-Based Research Tips & Tricks to Multiplexing: How to Choose Chromogen Colors for Multiplex and Detection Systems for Multiplex Assays Rhian Evans Ph.D., Scientist Multiplexing is an important tool for simultaneous detection of multiple markers within a single tissue section. Watch our 2 short videos below to learn more about multiplexing and download the complementary infographics to keep the points on hand when you need to revisit the information! How to choose chromogen colors for multiplex If you have opted for chromogenic multiplex staining, there are additional points to consider: the choice of colors and the order of color application. While this video won’t tell you exactly what, when, and where to use different chromogens, it will provide you with some tips to consider. Check out the infographic below and learn with Larry from Leica Biosystems about the tips to consider for chromogenic multiplex staining into your tissue-based research. Image DOWNLOAD Detection systems for multiplex assays There are many decisions that must be made when adopting multiplex staining, such as choosing chromogenic or immunofluorescent multiplex assays, as well as the order and colors of chromogens and fluorophores. However, the decisions don’t stop here as there are different detection systems available for multiplex assays that can increase sensitivity and enhance the signal-to-noise ratio when detecting markers of interest. We will cover a few of these examples in this video. Unsure of what detection systems are available for multiplex assays? Check out the infographic below that showcases some examples of detection systems available for multiplex assays that can increase sensitivity and enhance the signal-to-noise ratio when detecting markers of interest. Image DOWNLOAD DON'T MISS OUT! SUBSCRIBE TO RESEARCH LINK TODAY TO GET QUATERLY UPDATES ON OUR LATEST LIFE SCIENCE ENDEAVOURS SUBSCRIBE For research use only. Not for use in diagnostic procedures. About the presenter Rhian Evans , Ph.D., Scientist Rhian is a Scientist from Swansea University in Medical and Healthcare Studies and was featured in several collaborative publications. Rhian’s research-based background focused on tissue-based pathology in Multiple Sclerosis, primarily using immunohistochemical analysis and in vitro molecular techniques. She spent a short period conducting routine PCR testing for COVID-19 at the end of 2020. Related Content Selecting the right detection for your IHC/ISH project (Fluorescence vs Chromogenic Staining) Design, Optimization, and Validation of Multiplex Immunofluorescence Panels Benefits Of Automation For Tissue-Based Research Leica Biosystems content is subject to the Leica Biosystems website terms of use, available at: Legal Notice. The content, including webinars, training presentations and related materials is intended to provide general information regarding particular subjects of interest to health care professionals and is not intended to be, and should not be construed as, medical, regulatory or legal advice. The views and opinions expressed in any third-party content reflect the personal views and opinions of the speaker(s)/author(s) and do not necessarily represent or reflect the views or opinions of Leica Biosystems, its employees or agents. Any links contained in the content which provides access to third party resources or content is provided for convenience only. For the use of any product, the applicable product documentation, including information guides, inserts and operation manuals should be consulted. Copyright © 2024 Leica Biosystems division of Leica Microsystems, Inc. and its Leica Biosystems affiliates. All rights reserved. LEICA and the Leica Logo are registered trademarks of Leica Microsystems IR GmbH. Contact Our Product Expert Now If you have viewed this educational webinar or training and would like to apply for continuing education credits with your certifying organization, please download the form to assist you in adding self-reported educational credits to your transcript. Apply for self-reported educational credits Subscribe to our mailing list to learn about our upcoming symposiums and new research-focused product launches. Abonnez-vous aujourd'hui ! SHARE Facebook Twitter LinkedIn Email
