Developing Antibodies for Immunohistochemistry for Diagnosis in Formalin Fixed, Paraffin Embedded Tissues

Kay Savage
Kay Savage M.D., PhD

The objectives of the presentation is to give an overview of monoclonal antibody production, specifically to give antibodies of good technical quality for diagnostic immunohistochemistry on formalin fixed paraffin embedded material. Methods of clone selection, antibody characterization and protocol optimization will be discussed.

Learning Objectives

  1. Review of principles of immunohistochemistry
  2. Description of how monoclonal antibodies are made and how clones are selected
  3. Overview of verification and validation testing performed on selected clones
  4. Description of how product claims are generated

About the presenter

Kay Savage , M.D., PhD

Kay Savage currently works at at Leica Biosystems Newcastle, as a Principal Scientist in the Antibody Development group. Her main responsibilities are subject matter expert in immunohistochemistry, responsible for development and characterization of antibody products, and generation of product claims. Dr. Savage previously worked for over ten years in the Histopathology department at the Royal Free Hospital in London, where she worked on her PhD in pathology on the pathology of Hepatitis C virus, as well as other immunohistochemistry based research projects on melanomas, colon and gastric cancer. Her previous role was five years as senior scientist then laboratory manager at the pathology core facility for Breakthrough Breast Cancer at the Institute of Cancer Research in London, where her main responsibilities were optimization of novel antibodies for use on FFPE tissues, production of tissue microarrays for use in breast cancer research, and providing a histological service to the research institute.

Leica Biosystems Knowledge Pathway content is subject to the Leica Biosystems website terms of use, available at: Legal Notice. The content, including webinars, training presentations and related materials is intended to provide general information regarding particular subjects of interest to health care professionals and is not intended to be, and should not be construed as, medical, regulatory or legal advice. The views and opinions expressed in any third-party content reflect the personal views and opinions of the speaker(s)/author(s) and do not necessarily represent or reflect the views or opinions of Leica Biosystems, its employees or agents. Any links contained in the content which provides access to third party resources or content is provided for convenience only.

For the use of any product, the applicable product documentation, including information guides, inserts and operation manuals should be consulted.

Copyright © 2024 Leica Biosystems division of Leica Microsystems, Inc. and its Leica Biosystems affiliates. All rights reserved. LEICA and the Leica Logo are registered trademarks of Leica Microsystems IR GmbH.

If you have viewed this educational webinar, training or tutorial on Knowledge Pathway and would like to apply for continuing education credits with your certifying organization, please download the form to assist you in adding self-reported educational credits to your transcript.
Apply for self-reported educational credits
将 Knowledge Pathway 更新直接发送到您的收件箱。
立即订阅!
您的作品也能展示在这里！点击提交，期待与您合作！
Send your writing!
SHARE