Automated TRCBC1 and TRBC2 BaseScope™ as a Novel, Quicker and Cheaper Method for the Diagnosis of T-Cell Lymphoma Dr Elizabeth Soilleux Associate Professor, Honorary Consultant Pathologist and Research Group Leader, Dept of Pathology, University of Cambridge While assessment of B-cell populations for monotypia (kappa or lambda light chain restriction) as a surrogate for monoclonality, and thus likely malignancy (lymphoma or leukaemia), is well understood, an analogous approach for suspected T-cell lymphoma does not exist in routine clinical practice. At present, cases of suspected T-cell lymphoma undergo T-cell receptor clonality studies, which introduce clinical delay, are costly and require highly trained staff for interpretation, as well as losing any morphological or immunophenotypic context of clonal populations of cells. The University of Cambridge: · Undertook a pilot study in T-cell lymphomas and corresponding benign samples on the BOND RX research stainer, using BaseScope™ probes located in the highly divergent 3’UTRs of TRBC1 and TRBC2. · Showed that accurate diagnosis of T-cell lymphomas could be achieved in the majority of cases with this method. · Obtained similar results for the companion animals, dog and cat, in which the clinical question of T-cell lymphoma arises more frequently than in humans. Data will be presented indicating the potential utility of this approach in clinical practice. For Research Use Only. Not for use in diagnostic procedures. About the presenter Dr Elizabeth Soilleux , Associate Professor, Honorary Consultant Pathologist and Research Group Leader, Dept of Pathology, University of Cambridge Having undertaken her training and subsequently worked in both Oxford and Cambridge, Liz Soilleux is an Associate Professor, Honorary Consultant Pathologist and Research Group Leader in the Department of Pathology, University of Cambridge. She spends half of her time contributing to Cambridge’s large regional Haematopathology and Oncology Diagnostic Service, diagnosing lymphomas and leukaemias. She also undertakes molecular reporting (including of T and B-cell clonality studies) in the East Genomic Laboratory Hub, the largest of the UK’s genomic laboratory hubs. Her research interests lie in the use of harnessing aspects of T-cell receptor biology in diagnostics and in using machine learning approaches to advance diagnostic practice. Related Content Combining RNAscope™ Technology with Protein Detection: A Multi Omics approach using RNA-Protein Integrated Co-Detection Workflow (ICW) Incorporation and Automation of RNAscope Technology Into Your COVID-19 Viral Pathogenesis Research Leica Biosystems content is subject to the Leica Biosystems website terms of use, available at: Legal Notice. The content, including webinars, training presentations and related materials is intended to provide general information regarding particular subjects of interest to health care professionals and is not intended to be, and should not be construed as, medical, regulatory or legal advice. The views and opinions expressed in any third-party content reflect the personal views and opinions of the speaker(s)/author(s) and do not necessarily represent or reflect the views or opinions of Leica Biosystems, its employees or agents. Any links contained in the content which provides access to third party resources or content is provided for convenience only. For the use of any product, the applicable product documentation, including information guides, inserts and operation manuals should be consulted. Copyright © 2024 Leica Biosystems division of Leica Microsystems, Inc. and its Leica Biosystems affiliates. All rights reserved. LEICA and the Leica Logo are registered trademarks of Leica Microsystems IR GmbH. RELATED PRODUCTS BOND RX Fully Automated Research Stainer RELATED TAGS AND TOPICS T-cell lymphoma RELATED CONTENT Combining RNAscope™ Technology with Protein Detection: A Multi Omics approach using RNA-Protein Integrated Co-Detection Workflow (ICW) Incorporation and Automation of RNAscope Technology Into Your COVID-19 Viral Pathogenesis Research Contact Our Product Expert Now If you have viewed this educational webinar or training and would like to apply for continuing education credits with your certifying organization, please download the form to assist you in adding self-reported educational credits to your transcript. Apply for self-reported educational credits Subscribe to our mailing list to learn about our upcoming symposiums and new research-focused product launches. 立即订阅! SHARE Facebook Twitter LinkedIn Email
